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Plant tissue culture involves excising plant tissues and growing them on nutrient media. It is used rather broadly to include several variations, such as meristem culture for propagation of virus-free plants, protoplast culture, cell suspension culture, tissue and organ culture, and anther or pollen culture for producing haploid plants. This chapter focuses on various technical aspects of plant tissue culture. A suitable explant is selected and prepared for culture, and later incubated on an appropriate nutrient medium for growth and differentiation.
During plant tissue culture growth sucrose acts as a fuel source for sustaining photomixotrophic metabolism (organisms can use different sources of energy and carbon), ensuring optimal development, although other important roles such as carbon precursor or signaling metabolite have more recently been highlighted. Sucrose is a very important part of nutrient medium as an energy source, since most plant cultures are unable to photosynthesize effectively owing to poorly developed cellular and tissue development, lack of chlorophyll, inadequate gas exchange and carbon dioxide in tissue culture vessels, etc.
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The most important part of this activity, however, is to maintain as sterile an environment as possible. Even one fungal spore or bacterial cell that comes into contact with the growth medium will rapidly reproduce and soon completely overwhelm the small plant piece that you are trying to clone. If you wish to use plants other than cauliflower, you need to prepare two different media which contain plant hormones necessary to stimulate development of differentiated tissues. The first one should contain a cytokinin such as BAP which promotes shoot formation and the second one a rooting hormone such as NAA or store bought rooting hormone. To do this, prepare the mixture up until the end of step 2. Keeping the mixture warm so that it does not solidify, divide it equally into two pre-warmed containers.
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Foshan Youngplants Co., Ltd has grown into a large biotechnological known as ‘The Rising Star in the Field of Tissue Culture’, with 6 specialized in vitro tissue culture laboratories covering a total area of more than 11,000㎡. After 14 years’ efforts, our company has specialized in supplying in vitro tissue culture plants to both local and worldwide markets, and has eventually increased yearly output to over 80 million with stable quality and rich varieties. With the helps and supports of our global clients, professors and officials, we gained a strong reputation.
